Page Byline
LIFECODES Fluoroanalyzer
Accuracy and Precision
- Sample uptake volume ±5%
- Classification of microspheres >80%
- Misclassification of microspheres ≤ 2%.
- Temperature control 0°C to +2°C of target
- Internal sample carryover <0.9%
- Soluble background fluorescence emissions at 575 nm automatically subtracted from fluorescence intensity values
Sensitivity
- Detect 1000 fluorochromes phycoerythrin (PE) per xMAP microsphere
- Reporter channel dynamic range: 3.5
Optics
- Reporter laser: 532 nm, nominal output 10- 15 mW, maximum 500 mW, frequencydoubled diode; mode of operation, continuous wave (CW)
- Classification laser: 635 nm, 9.1 ± 6%, maximum output 25 mW, diode; mode of operation, continuous wave (CW)
- Reporter detector: Photomultiplier tube, detection bandwidth of 565 – 585 nm
- Classification detector and doublet discriminator: Avalanche photo diodes with temperature compensation
Fluidics
- Sheath flow rate: 90 μL ± 5 μL/second
- Cuvette: 200 micron square flow channel
- Sample injection rate: 1 μL/second
- Sample update volume: 20 – 200 μL
Setup
- Installation < 4 hours
- System calibration < 10 minutes
- System warm up 30 minutes. Systems that remain inactive for at least four hours will require a warm-up to restart the lasers. After acquiring sample, running system calibrators, running system controls and warming up the instrument, the system resets the four-hour internal clock.
Capacity The specifications below reflect minimum capacity values:
- Analyze multiple 96-well plates per batch
- Analyze multiple assay templates per plate
- Automatic sampling from a 96-well plate.
- Sheath container and waste container hold enough volume to run up to two 96-well plates between refills.
- Distinguish a minimum of 1 to a maximum of 100 unique xMAP microsphere sets in a single sample
- Detect and distinguish surface reporter fluorescence emissions at 575 nm on the surface of 1-100 unique xMAP
microsphere sets in a single sample
General
- Operating temperature: 15°C to 30°C (59°F to 86°F)
- Humidity: 20% to 80%, noncondensing
- Altitude: Operation up to 2400 m (7874 ft.) above mean sea level
- Physical dimensions: 43 cm (17 inches) W x 50.5 cm (20 inches) D x 24.5 cm (9.5inches) H
- Weight: 25 kg (60 lbs.)
- UL Installation Category II, as defined in Annex J of the UL 61010A-1
- UL Pollution Degree 2, as defined in Section 3.7.3.2 of UL 61010A-1
- Luminex 200 analyzer, input voltage range:
- 100 - 120 V~ ±10%, 1.4 Amp, and 200 -
- 240 V~ ±10%, 0.8 Amp, 47 – 63 Hz
Monitor Specifications
- Screen resolution and number of colors:
- SXGA 1280 x 1024 with 32-bit color
PC Specifications
- Processor: 1.86 GHz Intel® Core™2 or higher (2.8 GHz recommended)
- Main memory: 2 GB RAM or higher (4GB recommended)
- Hard disk drive: 80 GB
- Analyzer communication: one USB 1.1 compatible port
- XY Communication: one Luminex compatible RS232 port (StarTech serial port card, model PCI2S950DV)
- External Drive: DVD
- Operating system: English USA Microsoft® Windows® SP3 or English USA Windows® 7, 32-bit (not 64-bit)
- CE marked and UL listed
- Two-button mouse or equivalent
LIFECODES Fluoroanalyzer
Includes: LX200 analyzer, XY Luminex platform, PC with Luminex xPONENT® software
| | 888300/888302 |
LIFECODES Fluoroanalyzer Sheath Delivery System
Sheath Delivery System
| | 888305 |
The Luminex 200 System has been 510(k) cleared by the FDA. Kits developed by Luminex strategic partners that have received 510(k) clearance from the FDA can be used on the Luminex 200 system for diagnostic purposes. The Luminex 200 System is also CE marked for the EU.
LUMINEX is a trademark of Luminex Corporation. Accuracy and Precision - Sample uptake volume ±5%
- Classification of microspheres >80%
- Misclassification of microsphere <2%
- May vary by xMAP microsphere product lines. Refer to the specific product information sheet for further details
- Temperature control 0°C to +2°C of target
- Internal sample carryover <0.9%
- Soluble background fluorescence emissions at 575 nm automatically subtracted from fluorescence intensity values
Sensitivity
- Detects 1000 fluorochromes phycoerythrin (PE) per xMAP microsphere
- Reporter channel dynamic range: 3.5 decades of detection
Optics
- Reporter laser: 532 nm, nominal output 10-15 mW, maximum 500mW, frequency doubled diode; mode of operation, continuous wave (CW)
- Classification laser: 635 nm, 9.1 ±6%, maximum output 25 mW, diode; mode of operation, continuous wave (CW)
- Reporter detector: Photomultiplier tube, detection bandwidth of 565-585 nm
- Classification detector and doublet discriminator: Avalanche photo diodes with temperature compensation
- Fluidics
- Sheath flow rate: 90 µL ± 5 µL/second
- Cuvette: 200 micron square flow channel
- Sample injection rate: 1µL/second
- Sample update volume: 20-200 µL
Capacity
- The specifications below reflect minimum capacity values:
- Analyze multiple 96-well plates per batch
- Analyze multiple assay templates per plate
- Automatic sampling from a 96-well plate. The following microtiter plates are compatible with the Luminex XY Platform plate holder: flatbottom, conical, round, filter bottom, half plates [overall height no more than 0.75" (19mm)], any color
- Sheath container and waste container hold enough volume to run up to two 96-well plates between refills
- Distinguish a minimum of 1 to a maximum of 100 unique xMAP microsphere sets in a single sample
- Detect and distinguish surface reporter fluorescence emissions at 575 nm on the surface of 1-100 unique xMAP microsphere sets in a single sample
General
- Operating temperature: 15°C to 30°C (59°F to 86°F)
- Humidity: 20% to 80%, non-condensing
- Altitude: Operation up to 2400 m (7874 ft) above mean sea level
- Physical dimensions: 43 cm (17") W x 50.5 cm (20") D x 24.5 cm (9.5") H
- Weight: 25 kg (60 lbs.)
- UL Installation Category II, as defined in Annex J of the UL 61010A-1
- UL Pollution Degree 2, as defined in Section 3.7.3.2 of UL 61010A-1
- Luminex® 200 analyzer, input voltage range: 100-120V~±10%, 1.4 Amp, and 200-240 V~±10%, 0.8 Amp, 47-63 Hz
PC Specifications
- Intel Pentium IV or equivalent class PC, minimum speed 933 MHz
- Main memory: minimum of 512 MB
- Hard disk drive: 10 GB minimum storage capacity
- Communication/Ports: minimum of one parallel port, 2 RS-232 compatible serial ports, and one USB version 1.1 compatible high speed port
- Read, write and removable media: CDR/W drive
- Operating system: Microsoft Windows XP Pro SP2
- CE marked and UL listed
- Two-button mouse or equivalent
LUMINEX is a trademark of Luminex Corporation. INTEL and PENTIUM are trademarks of Intel Corporation. MICROSOFT and WINDOWS XP are trademarks of Microsoft Corporation.
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