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LIFECODES
Fluoroanalyzer​​​​​​

 

LIFECODES Fluoroanalyzer

 

Accuracy and Precision

  • Sample uptake volume ±5%
  • Classification of microspheres >80%
  • Misclassification of microspheres ≤ 2%.
  • Temperature control 0°C to +2°C of target
  • Internal sample carryover <0.9%
  • Soluble background fluorescence emissions at 575 nm automatically subtracted from fluorescence intensity values

Sensitivity

  • Detect 1000 fluorochromes phycoerythrin (PE) per xMAP microsphere
  • Reporter channel dynamic range: 3.5

Optics

  • Reporter laser: 532 nm, nominal output 10- 15 mW, maximum 500 mW, frequencydoubled diode; mode of operation, continuous wave (CW)
  • Classification laser: 635 nm, 9.1 ± 6%, maximum output 25 mW, diode; mode of operation, continuous wave (CW)
  • Reporter detector: Photomultiplier tube, detection bandwidth of 565 – 585 nm
  • Classification detector and doublet discriminator: Avalanche photo diodes with temperature compensation

Fluidics

  • Sheath flow rate: 90 μL ± 5 μL/second
  • Cuvette: 200 micron square flow channel
  • Sample injection rate: 1 μL/second
  • Sample update volume: 20 – 200 μL

Setup

  • Installation < 4 hours
  • System calibration < 10 minutes
  • System warm up 30 minutes. Systems that remain inactive for at least four hours will require a warm-up to restart the lasers. After acquiring sample, running system calibrators, running system controls and warming up the instrument, the system resets the four-hour internal clock.

Capacity
The specifications below reflect minimum capacity values:

  • Analyze multiple 96-well plates per batch
  • Analyze multiple assay templates per plate
  • Automatic sampling from a 96-well plate.
  • Sheath container and waste container hold enough volume to run up to two 96-well plates between refills.
  • Distinguish a minimum of 1 to a maximum of 100 unique xMAP microsphere sets in a single sample
  • Detect and distinguish surface reporter fluorescence emissions at 575 nm on the surface of 1-100 unique xMAP
  • microsphere sets in a single sample

General

  • Operating temperature: 15°C to 30°C (59°F to 86°F)
  • Humidity: 20% to 80%, noncondensing
  • Altitude: Operation up to 2400 m (7874 ft.) above mean sea level
  • Physical dimensions: 43 cm (17 inches) W x 50.5 cm (20 inches) D x 24.5 cm (9.5inches) H
  • Weight: 25 kg (60 lbs.)
  • UL Installation Category II, as defined in Annex J of the UL 61010A-1
  • UL Pollution Degree 2, as defined in Section 3.7.3.2 of UL 61010A-1
  • Luminex 200 analyzer, input voltage range:
    • 100 - 120 V~ ±10%, 1.4 Amp, and 200 -
    • 240 V~ ±10%, 0.8 Amp, 47 – 63 Hz

Monitor Specifications

  • Screen resolution and number of colors:
    • SXGA 1280 x 1024 with 32-bit color

PC Specifications

  • Processor: 1.86 GHz Intel® Core™2 or higher (2.8 GHz recommended)
  • Main memory: 2 GB RAM or higher (4GB recommended)
  • Hard disk drive: 80 GB
  • Analyzer communication: one USB 1.1 compatible port
  • XY Communication: one Luminex compatible RS232 port (StarTech serial port card, model PCI2S950DV)
  • External Drive: DVD
  • Operating system: English USA Microsoft® Windows® SP3 or English USA Windows® 7, 32-bit (not 64-bit)
  • CE marked and UL listed
  • Two-button mouse or equivalent